Primer Sequence 5-3 - 26 primeri -cunatificate de 2x ori prin spectrometrie UV-VIS - control de calitate QC 100% prin spectrometrie de masa LCMS - format lichid ready to use 100uM, nu necesita resuspendare - aprox 3500 reactii (conc 500nM la un vol total de 20ul per reactie) - scala de sinteza 100nmols – conc finala 25-35nMols - purificare standard purification desalting – high purity aroCfor CCTGGCACCTCGCGCTATAC aroCrev...

Primer Sequence 5-3 - 26 primeri -cunatificate de 2x ori prin spectrometrie UV-VIS - control de calitate QC 100% prin spectrometrie de masa LCMS - format lichid ready to use 100uM, nu necesita resuspendare - aprox 3500 reactii (conc 500nM la un vol total de 20ul per reactie) - scala de sinteza 100nmols – conc finala 25-35nMols - purificare standard purification desalting – high purity aroCfor CCTGGCACCTCGCGCTATAC aroCrev CCACACACGGATCGTGGCG aroCsfor GGCACCAGTATTGGCCTGCT aroCsrev CATATGCGCCACAATGTGTTG dnaNfor ATGAAATTTACCGTTGAACGTGA dnaNrev AATTTCTCATTCGAGAGGATTGC dnaNsfor CCGATTCTCGGTAACCTGCT dnaNsrev CCATCCACCAGCTTCGAGGT hemDfor2 GAAGCGTTAGTGAGCCGTCTGCG hemDrev2 ATCAGCGACCTTAATATCTTGCCA hemDsfor2 GTGGCCTGGAGTTTTCCACT hemDsrev2 GACCAATAGCCGACAGCGTAG hisDfor GAAACGTTCCATTCCGCGCAGAC hisDrev CTGAACGGTCATCCGTTTCTG hisDsfor GTCGGTCTGTATATTCCCGG hisDsrev GGTAATCGCATCCACCAAATC purEfor ATGTCTTCCCGCAATAATCC purErev TCATAGCGTCCCCCGCGGATC purEsfor CGCATTATTCCGGCGCGTGT purEsrev CGCGGATCGGGATTTTCCAG sucAsfor AGCACCGAAGAGAAACGCTG sucAsrev2 GGTTGTTGATAACGATACGTAC thrAfor GTCACGGTGATCGATCCGGT thrArev CACGATATTGATATTAGCCCG thrAsfor ATCCCGGCCGATCACATGAT thrAsrev CTCCAGCAGGGGCTCTTTCAG Master mix sonde qPCR - RealQ Plus Master Mix 400 rxn x 25ul Optimized All-in-one Master Mix for qPCR, well suited for quantitation, detection of gene expression, SNP analysis, multiplex PCR (for probe), and pathogen detection. 4x1,25m OneStep master mix (RNA to cDNA) RealQ Plus One-

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