2x 69104 DNeasy Plant Mini Kit, 50 preps.
1x 04-12-00125 5x FIREPol Master Mix Ready to Load with 12.5 mM MgCl2, 1 ml for 250 rxn.
1x 51235 AccuGENE 1X TE Buffer, 500 ml
1x A4150.0500 CTAB, lysis buffer, 500 ml
1x J22638.AE Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure, 100 ml
1x J62786.AK EDTA (ethylenediamine tetraacetic acid) 0.5 M in aqueous solution pH 8.0, 250 ml
1x P8107S Protein...
2x 69104 DNeasy Plant Mini Kit, 50 preps.
1x 04-12-00125 5x FIREPol Master Mix Ready to Load with 12.5 mM MgCl2, 1 ml for 250 rxn.
1x 51235 AccuGENE 1X TE Buffer, 500 ml
1x A4150.0500 CTAB, lysis buffer, 500 ml
1x J22638.AE Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure, 100 ml
1x J62786.AK EDTA (ethylenediamine tetraacetic acid) 0.5 M in aqueous solution pH 8.0, 250 ml
1x P8107S Proteinase K, Molecular Biology Grade, 800 units/ml, protein conc. 20 mg/ml, 2 x 1 ml
1x J62417.22 Polyvinylpyrrolidone, M.W. ∼ 40,000, 100 g
1x 77617-100ML Phenol – chloroform – isoamyl alcohol mixture, BioUltra, for molecular biology, 25:24:1, 100 ml
1x 27650.292 Sodium acetate ≥98%, GPR RECTAPUR, 1 kg
1x 27800.291 Sodium chloride ≥99%, GPR RECTAPUR, 1 kg
Pachet primeri ce contine secventele:
VVS2 Fw 5'- CAGCCCGTAAATGTATCCATC -3'
VVS2 Rev 5'- AAATTCAAAATTCTAATTCAACTGG -3'
VVMD5 Fw 5'- CTAGAGCTACGCCAATCCAA -3'
VVMD5 Rev 5'- TATACCAAAAATCATATTCCTAAA -3'
VVMD7 Fw 5'- AGAGTTGCGGAGAACAGGAT -3'
VVMD7 Rev 5'- CGAACCTTCACACGCTTGAT -3'
VVMD25 Fw 5'- TTCCGTTAAAGCAAAAGAAAAAGG -3'
VVMD25 Rev 5'- TTGGATTTGAAATTTATTGAGGGG -3'
VVMD27 Fw 5'- GTACCAGATCTGAATACATCCGTAAGT -3'
VVMD27 Rev 5'- ACGGGTATAGAGCAAACGGTGT -3'
VVMD28 Fw 5'- AACAATTCAATGAAAAGAGAGAGAGAGA -3'
VVMD28 Rev 5'- TCATCAATTTCGTATCTCTATTTGCTG -3'
VVMD32 Fw 5'- TATGATTTTTTAGGGGGGTGAGG -3'
VVMD32 Rev 5'- GGAAAGATGGGATGACTCGC -3'
VrZAG62 Fw 5'- GGTGAAATGGGCACCGAACACACGC -3'
etc
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