1. Testari imunoenzimatice,
Testari imunoenzimatice
1 MTT assay Kit, 1000 teste kit 2
2 Neutral Red Assay Kit , 1000 teste kit 1
3 Senescence β-Galactosidase Staining Kit Kit 1
4 DMF (Dimethylformamide) Buc 1
5 IFN gamma Human buc 1
6 TNF alpha Human buc 1
7 Acridine Orange Solution Buc 1
8 Ethidium Bromide Solution Buc 1
9 Anticorpi primari mono/policlonali buc 6
10 Anticorpi secundari buc 5
11 Beta Galactosid...
1. Testari imunoenzimatice,
Testari imunoenzimatice
1 MTT assay Kit, 1000 teste kit 2
2 Neutral Red Assay Kit , 1000 teste kit 1
3 Senescence β-Galactosidase Staining Kit Kit 1
4 DMF (Dimethylformamide) Buc 1
5 IFN gamma Human buc 1
6 TNF alpha Human buc 1
7 Acridine Orange Solution Buc 1
8 Ethidium Bromide Solution Buc 1
9 Anticorpi primari mono/policlonali buc 6
10 Anticorpi secundari buc 5
11 Beta Galactosidase Assay Kit kit 1
12 Hidrogen peroxide (Hydrogen peroxide 30% Suprapur®) buc 1
13 Doxorubicin Hydrochloride buc 1
14 Camptothecin buc 1
15 Paclitaxel buc 1
16 Medicarpin buc 1
17 Tert - Butyl hydroxiperoxide (tert-Butyl hydroperoxide solution 5.0-6.0 M in decane) buc 1
18 5- Bromouracil (5-Bromouracil 98%) buc 1
19 Hidroperoxid de hidrogen, 100 Ml fl 3
20 DCFDA / H2DCFDA- Cellular ROS Assay Kit, 300 teste buc 2
21 Lipid Peroxidation (MDA) Assay kit (colorimetric) 200 teste buc 1
22 Fura-2 Calcium Flux Assay Kit –No Wash, ratiometric 10 x 96 teste buc 1.
5. Consumabile pentru teste de laborator,
Consumabile pentru teste de laborator
1 Vacutainer EAB capac Verde, 17 UI/ml Litiu Heparina, cu aditiv, volum aspiratie 6ml, tub sticla 13x100mm, steril, 100 bucati/pachet pac 3
2 Vacutainer Hematologie, capac violet, cu anticoagulant K3 EDTA, volum aspiratie 3ml, 13x75mm, steril, 100 bucati/pachet pac 3
3 Manusi examinare nitril, fara pudra, Nitrylex Basic S, 100buc cutie 2
4 Manusi examinare nitril, fara pudra, Nitrylex Basic M, 100buc cutie 5
5 Manusi examinare nitril, fara pudra, Nitrylex Basic L, 100buc cutie 2
6 Halat chirurgical steril, marimea L buc 10
7 Flacoane culturi celulare, plastic, dop filetat cu filtru, 25 cm2, 200 buc/pachet pac 1
8 Placi sterile culturi celulare din polistiren, 96 godeuri, 100 buc pac 2
9 Placi sterile culturi celulare din polistiren, 24 godeuri, 100 buc pac 2
10 Placi pt culturi celulare 48 godeuri, 100 buc/pachet pac 1
11 Placi de cultura sterile, 6 godeuri pac 1
12 Lame cu 4 godeuri pentru culture celulare, 15 buc/pachet/ A chambered coverslip with 4 wells for use in munofluorescence and high-end microscopy pac 5
13 Tuburi centrifuga aseptice, fund conic 15 ml, 50 buc/pachet pac 10
14 Tuburi centrifuga aseptice, fund conic 50 ml, 50 buc/pachet pac 8
15 Criotuburi cu filet premium line, 2 ml sterile cu zona de scriere, 100 buc/pachet pac 2
16 Criotuburi cu filet interior si garnitura , 4 ml, 100 buc/pachet pac 2
17 Rezervor nesteril reactivi 50 ml, 10 buc/pachet pac 3
18 Pipete Pasteur cu capacitatea de 3 ml, ambalate individual, sterile, 100 buc/pachet pac 1
19 Pipete serologice sterile, gradate, ambalate individual 5 ML, 100 buc pac 1
20 Pipete serologice sterile, gradate, ambalate individual 10 ML, 100 buc pac 2
21 Pipete serologice sterile, gradate, ambalate individual 25 ML, 100 buc pac 1
22 Mikrozid AF Liquid cu pulverizator, 250 ml fl 3
23 Mikrozid AF servetele - rezerva de 150 serveţele buc 2
24 Mikrozid, cu pulverizator, 1 litru/fl fl 4
25 Slide-uri pentru teste de imunofluorescenta culturi celulare (Nunc Lab Tek II Chamber Slide System Sterile)- 154534 pac 2
26 Microtuburi sterile din polipropilena Eppendorf Safe-Lock, 2 ml, 100 bucati pac 1
27 Placi de cultura, 35x10mm Dish, Nunclon™ Delta (500 buc/ pac) pac 2
28 Folie Parafilm M ISOLAB, 75 m*50 mm buc 1
29 Etichete Sekuroka ROTH, Ø9.5 mm buc 1
30 Etichete crio ROTH, 33*13 mm buc 1
31 Anse pentru inoculare ISOLAB, 1 - 10 µl, 10 buc buc 10
32 Suport tuburi PCR ROTH, 76*76*25 mm, 5 buc buc 2
33 Criotuburi sterile din polipropilena ROTH, 2 ml, 200 buc/set set 3
34 Stativ albastru pentru criotuburi ISOLAB, 100 locasuri buc 2
35 0.2 ml 8-Tube PCR Strips without Caps, low profile, white (120 PCS) set 2
36 Hard-Shell 96-well skirted plates with white shell and white wells set 2
37 0.2mL PCR White plate for CFX96 cutie 2
38 Optical flat 8-cap strips, for 0.2 ml tubes and plates cutie 1
39 Microseal ‘B’ adhesive seals, optically clear cutie 1
40 Placute cromatografice din aluminiu cu silicagel (25 mm) 20x20 cm (silica gel 60 placă, suport din aluminiu, 20 * 20cm, cu indicator F254), 20 pcs/pk pk 2
41 Cutii Petri cu capac, 35x10 mm (1 pk) Greiner bio-one din plastic perfect transparent, nesterile pk 1
42 Syringe Filter, Cellulose Acetate, 0.20µm, 25mm, Pk / 100 pk 2
43 Tuburi nesterile pentru centrifuga, 23 ml, 400 buc pk 1
44 Suport tuburi centrifuga Isolab 15 - 50 mL buc 2.
4. Analize cromatografice,
Analize cromatografice
1 n-Hexan, 500 mL fl 2
2 Metanol, 2.5 L fl 10
3 Butanol sau n-butanol, 1 L fl 1
4 Acetat de etil, 1 L (Ethyl acetate for analysis EMSURE® ACS,ISO,Reag. Ph Eur) fl 1
5 Acid formic, 500 Ml (Formic acid ACS reagent, ≥96%) fl 2
6 Acetonitril, 5 L fl 10
7 (-)Epicatechina, 5 g fl 2
8 Rutin sau Quercetin 3-rhamnoglucosid, 100 mg fl 2
9 Quercetin dihidrate, 100 G fl 2
10 Acid cafeic sau acid 3,4-Dihidroxicinamic acid,, 25 g fl 1
11 Izorhamnetin sau , 10 mg fl 3
12 Luminol fl 3
14 Clorura de cobalt hexahidratata, 100 g fl 1
15 Acid trifluoracetic, 100 mL fl 2
16 butelie Azot 10L buc 10
17 morin hidrat, 10 g buc 2
18 Alcool etilic 95-96% denaturant buc 50
19 Alcool etilic absolut buc 50.
3. Analize de biologie moleculara,
Analize de biologie moleculara
1 TRIzol® Plus RNA Purification Kit (50 reaction) kit 4
2 Guanidine thiocyanate solution (50 ml/fl) fl 2
3 Ethanol for molecular biology 96-100% (250 ml/fl) fl 4
4 Chloroform for molecular biology (500 ml) fl 2
5 DNase I, RNase-free kit 2
6 Maxima First Strand cDNA Synthesis Kit for RT-qPCR (200 reaction) kit 1
7 Maxima Probe qPCR Master Mix (2X), with separate ROX vial (200 reaction) kit 1
8 NFkB-P65 Sens: (100 nmoli) 5¢ -ATGGACGATCTGTTTCCCCT-3¢ primer liofilizat 2
9 NFkB-P65 Anti-sens: (100 nmoli) 5¢ - CGGTTTACTCGGCAGATCTT-3¢ primer liofilizat 2
10 IkB-α Sens: (100 nmoli) 5¢ -GATGGCCTCAAGAAGGAGCGCT- 3¢ primer liofilizat 2
11 IkB-α Anti-sens: (100 nmoli) 5¢ -AGTGGAGATGCTGGGGTGTGCA- 3¢ primer liofilizat 2
12 β-actin Sens: (100 nmoli) 5¢ -GGTCTCAAACATGATCTGGG- 3¢ primer liofilizat 2
13 β-actin Anti-sens: (100 nmoli) 5¢ - GGGTCAGAAGGATTCCTATG- 3¢ primer liofilizat 2
14 IkB-α Sens: (100 nmoli) 5′-GCTGAAGAAGGAGCGGCTACT-3′ primer liofilizat 2
15 IkB-α Anti-sens: (100 nmoli) 5′-TCGTACTCCTCGTCTTT CATGGA-3′ primer liofilizat 2
16 GAPDH Sens: (100 nmoli) 5′- ATGGCACCGTCAAGGCTG-3′ primer liofilizat 2
17 GAPDH Anti-sens: (100 nmoli) 5′- GCAGTGATGGCATGGACTGT-3′ primer liofilizat 2
18 p53: Sens: (100 nmoli) 5′-TGCGTGTGGAGTATTTGGATG-3′ primer liofilizat 2
19 p53: Anti-sens: (100 nmoli) 5′-TGGTACAGTCAGAGCCAACCTC-3′ primer liofilizat 2
20 50 bp DNA Ladder buc 2
21 100 bp DNA Ladder buc 2
22 UltraPure™ Agarose 500G buc 1
23 UltraPure™ Ethidium Bromide, 10 mg/mL (1x10 ml) buc 1
24 TAE (10X), RNase-free buc 1
25 RT-PCR Grade Water (10x1.5 ml) buc 1
26 DEPC-Treated Water (1x100 ml) buc 1
27 BD Microtrol™ Salmonella typhimurium, ATCC 14028 (25 disc/tub) tub 1
28 CRYOINSTANT Mixed medium (50 tubes/pack) pac 1
29 Agar Luria (10 placi 10x90mm / pac pac 2
30 Bulion Luria (50 tuburi de 7ml /cutie) cutie 1
31 DNA AWAY™ Surface Decontaminant 250 ML fl 2
32 RNase AWAY™ Surface Decontaminant 250 ML fl 2
33 Luciferase Assay System with Reporter Lysis Buffer, 100 assays kit 2.
2. Intretinere culturi celulare,
Intretinere culturi celulare
1 Tripsina-EDTA pentru celule primare, 100 ml/flacon fl 4
2 Soluţie de neutralizare a tripsinei, 100 ml/flacon fl 4
3 Xeno-Free 0.05% Trypsin/0.02% EDTA Kit 100 mL kit 5
4 Histopaque-1077, 100 ml/flacon fl 2
5 LGM-3TM Lymphocyte Growth Medium-3, 500 ml/flacon fl 2
6 FGM™-2 Fibroblast Growth Medium-2 BulletKit kit 2
7 MEM Complete Medium, with NEAA, 1 mM sodium pyruvate, 2mM L-Glut, and 10% FBS, 500 ml/flacon fl 1
8 Trypan Blue, 0.4% Solution,liquid, sterile -filtered,suitable for cell culture, 100 ml fl 1
9 Ser fetal bovin, 100 ml fl 2
10 Penicillin − Streptomycin − Neomycin Solution Stabilized, 20 ml fl 2
11 Glicerol L 1
12 PBS, Phosphate Buffered Saline, 1X Solution, pH 7.4, Fisher BioReagents™ Buc 3
13 DULBECCO'S Phosphate Buffer Saline (PBS) fara calciu si magneziu, 500 ML fl 5
14 Enzyme Free Cell Dissociation Solution PBS Based (1X), liquid, 500ml fl 2
15 Acid Clorhidric – 1L Buc 3
16 NaOH- 1 kg (Sodium hydroxide pellets for analysis EMSURE®) Buc 2
17 Tampon fosfat salin pH 7.4, 50 tablete fl 6.
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